Genetic Factors in Radiation Resistance of Bacillus subtilis.

نویسندگان

  • S Zamenhof
  • H Bursztyn
  • T K Reddy
  • P J Zamenhof
چکیده

Zamenhof, Stephen (University of California, Los Angeles), Hela Bursztyn, T. K. Ramachandra Reddy, and Patrice J. Zamenhof. Genetic factors in radiation resistance of Bacillus subtilis. J. Bacteriol. 90:108-115. 1965.-A study of several wild cross-transformable strains of Bacillus subtilis revealed differences in the resistance of their spores to X rays. Closer study of two such strains revealed differences of the same type when vegetative cells were exposed to X rays or to ultraviolet light (UV). Cell cultures repeatedly exposed to sublethal doses of UV (with cultivation between exposures) became more resistant to UV, presumably by enrichment in a more UV-resistant mutant. A sulfanilamide-resistant mutant of one strain (vegetative cells and spores) was less resistant to ionizing radiation; this sensitivity was transferable by transformation. No difference in radiation-induced mutability could be demonstrated in any of the strains studied. It is concluded that, at least in the cases studied, (i) the differences in radiation resistance of spores of different strains are not just a result of a superimposition of a common spore resistance mechanism(s) but rather are an amplification of genetically determined resistance differences in vegetative cells of these strains; (ii) sulfanilamide-resistance locus (p-aminobenzoic acid overproduction locus) is one of the loci of radiation sensitivity; (iii) no evidence was obtained that the differences in radiation resistance of cells or spores can be ascribed to differences in radiation resistance of their deoxyribonucleic acid.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiation.

The role of DNA repair by nonhomologous-end joining (NHEJ) in spore resistance to UV, ionizing radiation, and ultrahigh vacuum was studied in wild-type and DNA repair mutants (recA, splB, ykoU, ykoV, and ykoU ykoV mutants) of Bacillus subtilis. NHEJ-defective spores with mutations in ykoU, ykoV, and ykoU ykoV were significantly more sensitive to UV, ionizing radiation, and ultrahigh vacuum than...

متن کامل

Decreased UV light resistance of spores of Bacillus subtilis strains deficient in pyrimidine dimer repair and small, acid-soluble spore proteins.

Loss of small, acid-soluble spore protein alpha reduced spore UV resistance 30- to 50-fold in Bacillus subtilis strains deficient in pyrimidine dimer repair, but gave only a 5- to 8-fold reduction in UV resistance in repair-proficient strains. However, both repair-proficient and -deficient spores lacking this protein had identical heat and gamma-radiation resistance.

متن کامل

Genetic characterization of recombination-deficient mutants of Bacillus subtilis.

Recombination-deficient (rec), radiation-sensitive mutations in Bacillus subtilis are grouped in at least seven distinct loci. Map positions are determined for six of these loci.

متن کامل

Effects of Mn levels on resistance of Bacillus megaterium spores to heat, radiation and hydrogen peroxide.

AIMS To determine the effects of Mn levels in Bacillus megaterium sporulation and spores on spore resistance. METHODS AND RESULTS Bacillus megaterium was sporulated with no added MnCl(2) and up to 1 mmol l(-1) MnCl(2). The resultant spores were purified and loosely bound Mn removed, and spore Mn levels were found to vary c. 100-fold. The Mn level had no effect on spore γ-radiation resistance,...

متن کامل

Effect of Coat Layers in Bacillus Subtilis Spores Resistance to Photo-Catalytic Inactivation

Different water treatment processes (physical and chemical) exist to obtain safe water for human or food industry supply. The advanced oxidation technologies are rising as a new alternative to eliminate undesirable chemicals and waterborne diseases. In this work, we analyze the power of the photo-assisted Fenton process using Fe(II)/H2O2 and UV radiation (365 nm) to inactivate Bacillus subtilis...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 90 1  شماره 

صفحات  -

تاریخ انتشار 1965